Difference between revisions of "Part:BBa K3002125"

 
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<partinfo>BBa_K3002125 short</partinfo>
 
<partinfo>BBa_K3002125 short</partinfo>
  
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This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the SP20-His-tag (BBa_K3002018), the secretion signal ARS (BBa_K3002009) and the coding sequence of the Wildtype-PETase (BBa_K3002004) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).  
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This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), the SP20-His-tag (<a href="https://parts.igem.org/Part:BBa_K3002018">BBa_K3002018</a>), the secretion signal ARS (<a href="https://parts.igem.org/Part:BBa_K3002009">BBa_K3002009</a>) and the coding sequence of the Wildtype-PETase (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>) plus the MoClo connectors for positions B1-B2 (<a href="https://parts.igem.org/Part:BBa_K3002302">BBa_K3002302</a>), B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:18, 22 October 2019


Level 1 Wildtype-PETase + ARS + sp20-His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the SP20-His-tag (BBa_K3002018), the secretion signal ARS (BBa_K3002009) and the coding sequence of the Wildtype-PETase (BBa_K3002004) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
    Illegal NgoMIV site found at 810
    Illegal NgoMIV site found at 837
    Illegal NgoMIV site found at 2539
  • 1000
    COMPATIBLE WITH RFC[1000]