Difference between revisions of "Part:BBa K3269004"
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This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part [[Part:BBa_K079050|BBa_K079050]]. For more information about our experience using this part and how the LexA 1 operator compares to the LexA 2 operator, please view the experience page and the page for part BBa_K079050.
 
This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part [[Part:BBa_K079050|BBa_K079050]]. For more information about our experience using this part and how the LexA 1 operator compares to the LexA 2 operator, please view the experience page and the page for part BBa_K079050.
  
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This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed.  
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This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed. Figure 2 shows the difference in GFP flurescence for part BBa_K079050 and part BBa_K3269004.  
  
 
https://static.igem.org/mediawiki/parts/b/be/T--Northwestern--lexA1data.png <br> Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004
 
https://static.igem.org/mediawiki/parts/b/be/T--Northwestern--lexA1data.png <br> Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004
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https://2019.igem.org/wiki/images/4/4a/T--Northwestern--LexA2vsLexA1.png
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Figure 2. Fluorescence/OD600 for parts BBa_K3269004 (LexA1) and BBa_K079050 (LexA2). The time value next to each title is the exposure time for each sample
 
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Revision as of 00:36, 22 October 2019


LexA1 GFP composite part

This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part BBa_K079050. For more information about our experience using this part and how the LexA 1 operator compares to the LexA 2 operator, please view the experience page and the page for part BBa_K079050.

This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed. Figure 2 shows the difference in GFP flurescence for part BBa_K079050 and part BBa_K3269004.

T--Northwestern--lexA1data.png
Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004

T--Northwestern--LexA2vsLexA1.png
Figure 2. Fluorescence/OD600 for parts BBa_K3269004 (LexA1) and BBa_K079050 (LexA2). The time value next to each title is the exposure time for each sample

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729