Difference between revisions of "Part:BBa K3046033"
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This part is a long sequence of DNA that are homologous to a part of the <i>albA</i> conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).
 
This part is a long sequence of DNA that are homologous to a part of the <i>albA</i> conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).
  
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 00:29, 22 October 2019


IS2 downstream sequence for A. niger genome integration

This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).

Usage and Biology

Characterization

We have demonstrated that the IS2 up- and downstream sequences work as expected qualitatively speaking. We did this by transforming a GFP reporter into Aspergillus niger ATCC1015, and as shown in figure 1, we were able to make the transformants express GFP as opposed to the wildtype control.

[[File:T--DTU-Denmark--GFPfung2.png]200px|fig.1|left|The two upper pictures show Aspergillus niger ATCC1015 transformed with the pPEA2P1 plasmid. The two pictures in the bottom show the same fungi, transformed with a self replicating AMA1 plasmid containing the PyrG marker]]



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 118
    Illegal BglII site found at 1360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
    Illegal NgoMIV site found at 1250
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1751
    Illegal SapI site found at 2060