Difference between revisions of "Part:BBa K2935073:Design"

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===Design Notes===
 
===Design Notes===
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No
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BBa_K2935073 (677bp) in saccharomyces cerevisiae chromosome S288C I position 205985-206661.
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In the genome of saccharomyces cerevisiae, most of the genes contain repeated sequences. Verstrepen,K.J. et al. [1] compared its effect on eight newly generated FLO1 alleles.It was found that as the Flo1 protein became longer (carrying more repeated sequences), the adhesion increased.Flocculation (i.e., adhesion to other yeast cells) showed a quantitative relationship between the same number of repetitions and the number of repetitions: the more repetitions, the greater the proportion of flocculated cells.The correlation between the number of repetitions observed and functional acquisition Flo1 depends on the specific amino acid encoded by the repeat sequence.
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To look for in-gene duplication, they used EMBOSS ETANDEMsoftware26 to screen all sacs ORFs sequences.Two separate screens screened short (3-39 nt) and long (440 nt) repeats, respectively.
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They ended up with BBa_K2935073 (677bp) as the basis and common piece of all FLO1.
  
 
===Source===
 
===Source===

Latest revision as of 00:26, 22 October 2019


Flo1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 121
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 121
    Illegal AgeI site found at 25
    Illegal AgeI site found at 67
    Illegal AgeI site found at 202
    Illegal AgeI site found at 472
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K2935073 (677bp) in saccharomyces cerevisiae chromosome S288C I position 205985-206661. In the genome of saccharomyces cerevisiae, most of the genes contain repeated sequences. Verstrepen,K.J. et al. [1] compared its effect on eight newly generated FLO1 alleles.It was found that as the Flo1 protein became longer (carrying more repeated sequences), the adhesion increased.Flocculation (i.e., adhesion to other yeast cells) showed a quantitative relationship between the same number of repetitions and the number of repetitions: the more repetitions, the greater the proportion of flocculated cells.The correlation between the number of repetitions observed and functional acquisition Flo1 depends on the specific amino acid encoded by the repeat sequence. To look for in-gene duplication, they used EMBOSS ETANDEMsoftware26 to screen all sacs ORFs sequences.Two separate screens screened short (3-39 nt) and long (440 nt) repeats, respectively. They ended up with BBa_K2935073 (677bp) as the basis and common piece of all FLO1.

Source

Chlamydomonas reinhardtii

References

[1]Intragenic tandem repeats generate functional variability.Verstrepen,K.J., Jansen,A., Lewitter,F. and Fink,G.R. Nat. Genet. 37 (9), 986-990 (2005)