Difference between revisions of "Part:BBa K079050"
Line 4: Line 4:
 
This part is a DNA damage reporter. See experience part for more Experimental details.
 
This part is a DNA damage reporter. See experience part for more Experimental details.
  
−
The Northwestern 2019 iGEM team provided novel characterization data to part BBa_K079050. The 2008 Bologna Team reported their part unable to be induced by UV light, however, after exposing cultures transformed with part BBa_K079050 to various doses of UV-C (0 J/m^2, 50 J/m^2, and 125 J/m^2) we found that their part was completely inducible by UV light. Additionally, the rate of decrease in Fluorescence/OD is significantly different between each exposure time, providing evidence that this part may actually be able to differentiate between different UV doses, just not in the way it was originally designed for (Graph below). Fluorescence measurements were taken every 3 minutes immediately after exposure in a 96-well plate reader for 3 hours.  
+
The Northwestern 2019 iGEM team provided novel characterization data to part BBa_K079050. The 2008 Bologna Team reported their part was unable to be induced by UV light. However, after exposing it to various doses of UV-C (0 J/m^2, 50 J/m^2, and 125 J/m^2), we found that their part showed different responses for different amounts of UV exposure. Specifically, the rate of decrease in Fluorescence/OD is significantly different between each exposure time, providing evidence that this part may actually be able to differentiate between different UV doses, just not in the way it was originally designed for (Figures 3 and 4). Fluorescence measurements were taken every 3 minutes immediately after exposure in a 96-well plate reader for 3 hours.
  
 
https://2019.igem.org/wiki/images/d/d0/T--Northwestern--LexA2FODgraph.png
 
https://2019.igem.org/wiki/images/d/d0/T--Northwestern--LexA2FODgraph.png
 +
<br>
 +
<b>Figure 3. Fluorescence/OD values for Part BBa_K079050 after exposure to various UV doses.</b> <br>
  
 +
https://2019.igem.org/wiki/images/5/53/T--Northwestern--lexa2fa.png" width = 550px
 +
<br>
 +
<b> Figure 4. Fold Activation for part Part BBa_K079050 after exposure to various UV doses. </b>
 +
<br>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 00:22, 22 October 2019

GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator

This part is a DNA damage reporter. See experience part for more Experimental details.

The Northwestern 2019 iGEM team provided novel characterization data to part BBa_K079050. The 2008 Bologna Team reported their part was unable to be induced by UV light. However, after exposing it to various doses of UV-C (0 J/m^2, 50 J/m^2, and 125 J/m^2), we found that their part showed different responses for different amounts of UV exposure. Specifically, the rate of decrease in Fluorescence/OD is significantly different between each exposure time, providing evidence that this part may actually be able to differentiate between different UV doses, just not in the way it was originally designed for (Figures 3 and 4). Fluorescence measurements were taken every 3 minutes immediately after exposure in a 96-well plate reader for 3 hours.

T--Northwestern--LexA2FODgraph.png
Figure 3. Fluorescence/OD values for Part BBa_K079050 after exposure to various UV doses.

T--Northwestern--lexa2fa.png" width = 550px
Figure 4. Fold Activation for part Part BBa_K079050 after exposure to various UV doses.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729