Difference between revisions of "Part:BBa K3208012"

Latest revision as of 00:21, 22 October 2019

Modified gRNA expression vector

This part is a modified version of part BBa_K3208000. This new gRNA expression vector contains red expression cassette (BBa_ K3208010) inserted between the two Eco31I sites. This allows selection of positive transformants based on colour when one uses these 2 sites for cloning of new gRNAs.

We performed 3 cloning experiments (with 3 different gRNAs that were given to us as overlapping oligos by team iGEM Bulgaria 2017) ) and we found that when using the right color as a selection tool, we increased the cloning efficiency to the range of 4-6 positive colonies per 10 white colonies. This is a huge improvement compared to the previous version (see part BBa_K2515002 for details).

This part is used as a Contribution by the iGEM Bulgaria 2019 team!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 8
Illegal NheI site found at 31
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 824
Illegal AgeI site found at 936
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1073
Illegal BsaI.rc site found at 38

Revision as of 00:11, 22 October 2019 (view source) Registry (Talk \| contribs)		Latest revision as of 00:21, 22 October 2019 (view source) Slavil (Talk \| contribs)
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	We performed 3 cloning experiments (with 3 different gRNAs that were given to us as overlapping oligos by team iGEM Bulgaria 2017) ) and we found that when using the right color as a selection tool, we increased the cloning efficiency to the range of 4-6 positive colonies per 10 white colonies. This is a huge improvement compared to the previous version (see part BBa_K2515002 for details).		We performed 3 cloning experiments (with 3 different gRNAs that were given to us as overlapping oligos by team iGEM Bulgaria 2017) ) and we found that when using the right color as a selection tool, we increased the cloning efficiency to the range of 4-6 positive colonies per 10 white colonies. This is a huge improvement compared to the previous version (see part BBa_K2515002 for details).
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		+	<b>This part is used as a Contribution by the iGEM Bulgaria 2019 team!</b>
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