Difference between revisions of "Part:BBa K3015000"
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Riboswitch specific for Mycolactone. Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch. | Riboswitch specific for Mycolactone. Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch. | ||
In the absence of Mycolactone the terminator-sequence will bind to the aptamer-sequence and form a hairpin, which will cause the RNA-Polymerase to dissociate from the DNA-strand. | In the absence of Mycolactone the terminator-sequence will bind to the aptamer-sequence and form a hairpin, which will cause the RNA-Polymerase to dissociate from the DNA-strand. | ||
| − | In the presence of Mycolactone it will bind to the Aptamer-sequence and loosen the hairpin structure and the RNA-Polymerase can transcribe. | + | In the presence of Mycolactone it will bind to the Aptamer-sequence and loosen the hairpin structure and the RNA-Polymerase can transcribe.<br> |
| + | [[File:T--BOKU-Vienna--K3015001mycolactone.png|600px]]<br> | ||
| + | Figure 1: Secondary structure of Mycolactone aptamer with intrinsic termination<br> | ||
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Revision as of 00:18, 22 October 2019
Mycolactone-Riboswitch
Riboswitch specific for Mycolactone. Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.
In the absence of Mycolactone the terminator-sequence will bind to the aptamer-sequence and form a hairpin, which will cause the RNA-Polymerase to dissociate from the DNA-strand.
In the presence of Mycolactone it will bind to the Aptamer-sequence and loosen the hairpin structure and the RNA-Polymerase can transcribe.
Figure 1: Secondary structure of Mycolactone aptamer with intrinsic termination
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]