Difference between revisions of "Part:BBa K2010999"
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This sequence is the E. coli K12 optimized DNA sequence for PETase. | This sequence is the E. coli K12 optimized DNA sequence for PETase. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 00:17, 22 October 2019
PETase (PET-degrading enzyme, origin I. sakaiensis)
PETase is a poly(ethylene terehphthalate)-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
<img src= "https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-pH-7_Wildtype-All-Conc.png" width="50%" height="50%">
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]