Difference between revisions of "Part:BBa K2010999"
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<partinfo>BBa_K2010999 short</partinfo>
 
<partinfo>BBa_K2010999 short</partinfo>
  
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PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis.
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PETase is a poly(ethylene terehphthalate)-degrading enzyme first identified in Ideonella sakaiensis.
 
This sequence is the E. coli K12 optimized DNA sequence for PETase.
 
This sequence is the E. coli K12 optimized DNA sequence for PETase.
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ATGAATTTTCCTCGCGCTTCACGACTGATGCAGGCTGCCGTTCTTGGAGGGCTGATGGCCGTTAGCGCGGCCGCCACCGCTCAGACAAATCCCTACGCCCGCGGTCCGAATCCGACAGCCGCCAGTTTGGAAGCGAGCGCTGGTCCATTCACCGTTCGCTCCTTTACCGTGAGTAGACCGAGCGGTTATGGCGCTGGCACCGTTTACTATCCAACAAATGCTGGGGGTACCGTGGGCGCCATAGCCATAGTTCCCGGGTATACGGCACGGCAGTCATCAATTAAATGGTGGGGACCGCGTCTGGCATCCCACGGTTTCGTAGTAATTACAATTGACACAAATTCCACGTTAGACCAGCCATCAAGTCGGAGTTCGCAACAAATGGCCGCGCTGCGCCAGGTGGCGTCGTTAAACGGTACAAGTAGCAGCCCGATTTACGGAAAGGTCGATACCGCTCGTATGGGTGTTATGGGGTGGAGTATGGGAGGTGGAGGCTCCCTGATCTCTGCTGCTAACAACCCTTCGCTGAAAGCAGCGGCGCCTCAAGCACCATGGGATTCTTCGACAAATTTTAGTTCTGTAACTGTGCCCACGCTGATCTTCGCATGTGAAAACGATAGTATAGCCCCGGTCAACTCTTCAGCACTTCCTATCTATGATTCTATGTCACGCAACGCTAAGCAGTTTCTCGAAATTAATGGTGGCTCACATTCCTGTGCGAATAGCGGCAATTCTAACCAAGCATTAATCGGAAAAAAAGGCGTTGCATGGATGAAACGTTTTATGGACAATGATACTAGGTATTCTACTTTTGCCTGCGAGAACCCGAATAGCACCAGAGTGTCTGATTTTCGTACAGCGAATTGCAGCTGA
 
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^ Updated 20191016 by Victoria Sajtovich of iGEM Toronto 2019 (due to the sequence's absence)
 
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iGEM HQ: see correspondence to William Cho and traci
 
  
  

Revision as of 00:11, 22 October 2019


PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is a poly(ethylene terehphthalate)-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]