Difference between revisions of "Part:BBa K157008"
(New page: ==Split-Venus-nYFP== N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba...) |
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==Split-Venus-nYFP== | ==Split-Venus-nYFP== | ||
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP “Venus”, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].<br><br> | N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP “Venus”, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].<br><br> | ||
| + | <partinfo>BBa_K157008 SequenceAndFeatures</partinfo> | ||
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002<br> | - [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002<br> | ||
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)<br> | - [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)<br> | ||
Revision as of 22:45, 26 October 2008
Split-Venus-nYFP
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP “Venus”, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
-[4]Nagai, T. et al. “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications” J. Biol. Chem. 276, 29188-29194, 2001
-[5]Roger Y. Tsien et al. „Creating new fluorescent probes for cell biology“, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002