Difference between revisions of "Part:BBa K3002103:Design"
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===Source=== | ===Source=== | ||
| − | This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002004 (Wildtype PETase), | + | This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002004 (Wildtype PETase), BBa_K3002017 (HA-tag), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6) |
===References=== | ===References=== | ||
Latest revision as of 23:48, 21 October 2019
Level 1 Wildtype PETase + His (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1002
Illegal PstI site found at 1975
Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 764
Illegal NgoMIV site found at 2415 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002004 (Wildtype PETase), BBa_K3002017 (HA-tag), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6)