Difference between revisions of "Part:BBa K2996500"
 
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<partinfo>BBa_K2996500 SequenceAndFeatures</partinfo>
 
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<center><img style="max-width: 80%" src="https://2019.igem.org/wiki/images/e/e2/T--SJTU-BioX-Shanghai--jsf-NDBD.png" alt="" /></center>
 
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<center><b>Figure 1.</b> <i>Fig.2 PCR product of DBD with rrnB T1 terminator downstream.(Lane1:product of DBD;Lane 2:2000bp ladder).</i> </center>
 
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2996500 parameters</partinfo>
 
<partinfo>BBa_K2996500 parameters</partinfo>
 
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Latest revision as of 23:42, 21 October 2019


Zinc finger with flag tag

It is a sequence encoding protein that can recruit zinc ions for functioning.The protein can bind specific DNA sequence 5'-aTTAGGAGTGa-3'.To better purify the protein,we also added a flag tag at the C terminal of it. It is composed of mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]