Difference between revisions of "Part:BBa K3117015"

 
Line 3: Line 3:
 
<partinfo>BBa_K3117015 short</partinfo>
 
<partinfo>BBa_K3117015 short</partinfo>
  
−
...
+
The SpyTag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins. It is used in the construct to recognize the corresponding molecule, Catcher, and form a covalent isopeptide bond between the side chains of an reactive aspartate and lysine in SpyCatcher (Hatlem et al. 2019).
 +
 
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
The SpyTag/SpyCatcher system is based on a modified domain from a streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag), which can bind spontaneously and form an isopeptide bond (Hatlem et al. 2019).
  
 
<!-- -->
 
<!-- -->

Revision as of 23:40, 21 October 2019


SpyTag codon optimized for CHO expression

The SpyTag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins. It is used in the construct to recognize the corresponding molecule, Catcher, and form a covalent isopeptide bond between the side chains of an reactive aspartate and lysine in SpyCatcher (Hatlem et al. 2019).


Usage and Biology

The SpyTag/SpyCatcher system is based on a modified domain from a streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag), which can bind spontaneously and form an isopeptide bond (Hatlem et al. 2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]