Difference between revisions of "Part:BBa K3269004"

Revision as of 23:40, 21 October 2019

LexA1 GFP composite part

This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part BBa_K079050. For more information about our experience using this part, please view the experience page and the page for part BBa_K079050.

This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed.

Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004 Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 729

@@ Line 5: / Line 5: @@
 This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part [[Part:BBa_K079050|BBa_K079050]]. For more information about our experience using this part, please view the experience page and the page for part BBa_K079050.
+This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed.
+https://static.igem.org/mediawiki/parts/b/be/T--Northwestern--lexA1data.png <br> Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004
 <!-- Add more about the biology of this part here
 ===Usage and Biology===