Difference between revisions of "Part:BBa K3317002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 23:33, 21 October 2019
Protocatechuate decarboxylase (aroY) from Klebsiella pneumonia subsp.
Protocatechuate decarboxylase (aroY gene) converted the protocatechuic acid into catechol. The enzyme was modified with a signal peptide at its N-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et. al 2017) This is a hexameric enzyme
Wild type enzyme information:
E. C. 4.1.1.63
Km 0.59
Kcat 9x10-5
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 55
Illegal AgeI site found at 381 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1045