Difference between revisions of "Part:BBa K3317003"

Revision as of 23:32, 21 October 2019

Catechol 1,2-dioxygenase (CatA) from Pseudomonas putida

Catechol 1,2-dioxygenase (CatA) converted catechol into cis,cis-muconic acid. The enzyme was modified with a signal peptide at its N-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et. al 2017) This is a dimeric enzyme

Wild type enzyme information: E. C. 1.13.11.1

Km 0.00185

Kcat 19.39725

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 283
Illegal NgoMIV site found at 355
Illegal AgeI site found at 55
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 967

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3317003 short</partinfo>
-Catechol 1,2-dioxygenase (CatA) converted catechol into cis,cis-muconic acid. The enzyme was modified with a signal peptide at its N-terminal () to be introduced into bacterial microcompatments (BMC)
+Catechol 1,2-dioxygenase (CatA) converted catechol into cis,cis-muconic acid. The enzyme was modified with a signal peptide at its N-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J <i> et. al</i> 2017)
+This is a dimeric enzyme
+<html><img style="padding: 0 15px; height: 200px; weight: 200px float: right;" src="https://2019.igem.org/File:T--UANL--circuit--cat_a.png"></html>
+Wild type enzyme information:
+E. C. 1.13.11.1
+Km 0.00185
+Kcat 19.39725
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