Difference between revisions of "Part:BBa K3317002"

Revision as of 23:22, 21 October 2019

Protocatechuate decarboxylase (aroY) from Klebsiella pneumonia subsp.

Protocatechuate decarboxylase (aroY gene) converted the protocatechuic acid into catechol. The enzyme was modified with a signal peptide at its N-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et. al 2017) This is a dimeric enzyme

Wild type enzyme information: E. C. 4.1.1.63

Km 0.59

Kcat 9x10-5 Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 55
Illegal AgeI site found at 381
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1045

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3317002 short</partinfo>
-Protocatechuate decarboxylase (aroY) converted the protocatechuic acid (PCB) into catechol. The enzyme was modified with a signal peptide at its N-terminal () to be introduced into bacterial microcompatments (BMC)
+Protocatechuate decarboxylase (aroY gene) converted the protocatechuic acid into catechol. The enzyme was modified with a signal peptide at its N-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J <i> et. al</i> 2017)
+This is a dimeric enzyme
+<html><img style="padding: 0 15px; height: 200px; weight: 200px float: right;" src="https://2019.igem.org/wiki/images/7/75/T--UANL--circuit--aroy.png"></html>
+Wild type enzyme information:
+E. C. 4.1.1.63
+Km 0.59
+Kcat 9x10-5
 <!-- Add more about the biology of this part here
 ===Usage and Biology===