Difference between revisions of "Part:BBa K3117012:Design"
Lena Schorr (Talk | contribs) |
Lena Schorr (Talk | contribs) |
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===Design Notes=== | ===Design Notes=== | ||
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| + | pelB was fused to the 5' end of the constructs for targeting the recombinant proteins to the periplasm. | ||
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===Source=== | ===Source=== | ||
| − | + | pelB originates from genomic DNA. | |
===References=== | ===References=== | ||
Latest revision as of 23:21, 21 October 2019
pelB signal sequence
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pelB was fused to the 5' end of the constructs for targeting the recombinant proteins to the periplasm.
Source
pelB originates from genomic DNA.
References
1. Singh, Pranveer; Sharma, Likhesh; Kulothungan, S. Rajendra; Adkar, Bharat V.; Prajapati, Ravindra Singh; Ali, P. Shaik Syed et al. (2013): Effect of signal peptide on stability and folding of Escherichia coli thioredoxin. In: PloS one 8 (5), e63442. DOI: 10.1371/journal.pone.0063442.
2. Schlegel, Susan; Rujas, Edurne; Ytterberg, Anders Jimmy; Zubarev, Roman A.; Luirink, Joen; Gier, Jan-Willem de (2013): Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels. In: Microbial cell factories 12, S. 24. DOI: 10.1186/1475-2859-12-24.