Difference between revisions of "Part:BBa K3317001"
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Vanillate O-demethylase (LigM)(<partinfo>K3317000</partinfo>), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et al. 2017) | Vanillate O-demethylase (LigM)(<partinfo>K3317000</partinfo>), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et al. 2017) | ||
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This dimeric enzyme is tetrahydrofolate-dependent. | This dimeric enzyme is tetrahydrofolate-dependent. | ||
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| + | <html><img style="padding: 0 15px; height: 200px; weight: 200px float: right;" src="https://2019.igem.org/wiki/images/3/35/T--UANL--parts--ligps.png"></html> | ||
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Wild type enzyme information: | Wild type enzyme information: | ||
E. C. 2.1.1.341 | E. C. 2.1.1.341 | ||
Revision as of 23:06, 21 October 2019
Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6 with the pduP signal peptide
Vanillate O-demethylase (LigM)(BBa_K3317000), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et al. 2017) This dimeric enzyme is tetrahydrofolate-dependent.
Wild type enzyme information:
E. C. 2.1.1.341
Km 0.078
Kcat 7.1292
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 369
Illegal NgoMIV site found at 1150 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1372