Difference between revisions of "Part:BBa K3117005"
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This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection.
 
This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection.
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3117005 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
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<partinfo>BBa_K3117005 parameters</partinfo>
 
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Revision as of 23:05, 21 October 2019


6xHis-Tag

This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection.