Difference between revisions of "Part:BBa K2971010"

 
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crtEB
 
crtEB
  
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The part supplies the precursors for the synthetic pathway of carotenoids.
+
This part contains the sequences of crtE and crtB connected by a short linker sequence. The stop codon
 +
of each gene has been removed to express the genes in a single open reading frame. The enzymes
 +
encoded by the genes supply the precursor of carotenoids when expressed in Escherichia coli.
  
 
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Revision as of 22:45, 21 October 2019


crtE and crtB in a single open reading frame

crtEB

This part contains the sequences of crtE and crtB connected by a short linker sequence. The stop codon of each gene has been removed to express the genes in a single open reading frame. The enzymes encoded by the genes supply the precursor of carotenoids when expressed in Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1602
    Illegal BamHI site found at 841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 43