Difference between revisions of "Part:BBa K135001"
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This is a fusion of the CpxR responsive promoter pCpxR (BBa_K135000), upstream of BBa_E0840. It is not shown below in simple composite form as there was an extra bp in the linker sequence between BBa_K135000 and BBa_E0840 that the composite tool put in, which was not there in sequencing. | This is a fusion of the CpxR responsive promoter pCpxR (BBa_K135000), upstream of BBa_E0840. It is not shown below in simple composite form as there was an extra bp in the linker sequence between BBa_K135000 and BBa_E0840 that the composite tool put in, which was not there in sequencing. | ||
− | This part can be used as a visual assay for adhesion to hydrophobic surfaces in E. | + | This part can be used as a visual assay for adhesion to hydrophobic surfaces in ''E. coli''. See [[Part:BBa_K135000 | part BBa_K135000]] for an explanation of how this works. |
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Revision as of 21:16, 26 October 2008
pCpxR-BBa_E0840 fusion
This is a fusion of the CpxR responsive promoter pCpxR (BBa_K135000), upstream of BBa_E0840. It is not shown below in simple composite form as there was an extra bp in the linker sequence between BBa_K135000 and BBa_E0840 that the composite tool put in, which was not there in sequencing.
This part can be used as a visual assay for adhesion to hydrophobic surfaces in E. coli. See part BBa_K135000 for an explanation of how this works.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 727