Difference between revisions of "Part:BBa K3317000"
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<partinfo>BBa_K3317000 short</partinfo> | <partinfo>BBa_K3317000 short</partinfo> | ||
− | Vanillate O-demethylase (LigM gene) converted the vanillate into protocatechuic acid and Tetrahydrofolate into 5-methyltetrahydrofolate .This enzyme is tetrahydrofolate-dependent. | + | Vanillate O-demethylase (LigM gene) converted the vanillate into protocatechuic acid and Tetrahydrofolate into 5-methyltetrahydrofolate . |
+ | |||
+ | This dimeric enzyme is tetrahydrofolate-dependent. | ||
E. C. 2.1.1.341 | E. C. 2.1.1.341 |
Latest revision as of 22:33, 21 October 2019
Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6
Vanillate O-demethylase (LigM gene) converted the vanillate into protocatechuic acid and Tetrahydrofolate into 5-methyltetrahydrofolate .
This dimeric enzyme is tetrahydrofolate-dependent.
E. C. 2.1.1.341
Km 0.078
Kcat 7.1292
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 369
Illegal NgoMIV site found at 1150 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1372