Difference between revisions of "Part:BBa K3317000"

 
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<partinfo>BBa_K3317000 short</partinfo>
 
<partinfo>BBa_K3317000 short</partinfo>
  
Vanillate O-demethylase (LigM) converted the vanillate into protocatechuic acid (PCB).This enzyme istetrahydrofolate-dependent.
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Vanillate O-demethylase (LigM gene) converted the vanillate into protocatechuic acid and Tetrahydrofolate into 5-methyltetrahydrofolate .This enzyme is tetrahydrofolate-dependent.
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E. C. 2.1.1.341
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Km 0.078
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Kcat 7.1292
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:30, 21 October 2019


Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6

Vanillate O-demethylase (LigM gene) converted the vanillate into protocatechuic acid and Tetrahydrofolate into 5-methyltetrahydrofolate .This enzyme is tetrahydrofolate-dependent.

E. C. 2.1.1.341 Km 0.078 Kcat 7.1292


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 369
    Illegal NgoMIV site found at 1150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1372