Difference between revisions of "Part:BBa K3317000"
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<partinfo>BBa_K3317000 short</partinfo> | <partinfo>BBa_K3317000 short</partinfo> | ||
− | Vanillate O-demethylase (LigM) converted the vanillate into protocatechuic acid | + | Vanillate O-demethylase (LigM gene) converted the vanillate into protocatechuic acid and Tetrahydrofolate into 5-methyltetrahydrofolate .This enzyme is tetrahydrofolate-dependent. |
+ | |||
+ | E. C. 2.1.1.341 | ||
+ | Km 0.078 | ||
+ | Kcat 7.1292 | ||
+ | |||
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Revision as of 22:30, 21 October 2019
Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6
Vanillate O-demethylase (LigM gene) converted the vanillate into protocatechuic acid and Tetrahydrofolate into 5-methyltetrahydrofolate .This enzyme is tetrahydrofolate-dependent.
E. C. 2.1.1.341 Km 0.078 Kcat 7.1292
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 369
Illegal NgoMIV site found at 1150 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1372