Difference between revisions of "Part:BBa K2943901"

Latest revision as of 22:24, 21 October 2019

Improved mRFP

This sequence is part of a composite part (BBa_K2943902) that aims to improve an existing composite part (BBa_K608014). The sequence is based on the monomer RFP (mRFP, BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were placed in order to optimize the mRNA folding energy (calculated by VienneRNA) to gain more efficient translation initiation and therefore higher expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 555
Illegal AgeI site found at 667
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2943901 short</partinfo>
-This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014).<br>
+This sequence is part of a composite part (BBa_K2943902) that aims to improve an existing composite part (BBa_K608014). The sequence is based on the monomer RFP (mRFP, BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were placed in order to optimize the mRNA folding energy (calculated by VienneRNA) to gain more efficient translation initiation and therefore higher expression.
-This sequence is based on the monomer RFP (mRFP, https://parts.igem.org/Part:BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were achieved by the program VienneRNA and optimize the mRNA folding energy to gain more efficient translation and expression.
 <!-- Add more about the biology of this part here