Difference between revisions of "Part:BBa K3192019"

Latest revision as of 22:23, 21 October 2019

styE

This part contains the styE gene taken from Pseudomonas putida S12 . This encodes the putative styrene transporter protein. This protein works to help shuttle the styrene molecules across the cell membrane into the cytoplasm to begin styrene degradation. It contains a 24 residue peptide for signaling to transport styrene. This is vital in the gene cluster styABCDE to allow the cell to metabolize styrene.¹

References:

1) Mooney, A., Oleary, N. D. & Dobson, A. D. W. Cloning and Functional Characterization of the styE Gene, Involved in Styrene Transport in Pseudomonas putida CA-3. Applied and Environmental Microbiology 72,1302–1309 (2006).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1040
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 125

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3192019 short</partinfo>
-This gene encodes styE, a gene essential for the biochemical pathway degrading styrene into phenyl acetic acid.
+<p>This part contains the styE gene taken from <i>Pseudomonas putida S12 </i>. This encodes the putative styrene transporter protein. This protein works to help shuttle the styrene molecules across the cell membrane into the cytoplasm to begin styrene degradation. It contains a 24 residue peptide for signaling to transport styrene. This is vital in the gene cluster styABCDE to allow the cell to metabolize styrene.<sup>1</sup> </p>
+<h3> References: </h3>
+<p> 1) Mooney, A., Oleary, N. D. & Dobson, A. D. W. Cloning and Functional Characterization of the styE Gene, Involved in Styrene Transport in Pseudomonas putida CA-3. <i> Applied and Environmental Microbiology </i> 72,1302–1309 (2006). </p>
 <!-- Add more about the biology of this part here