Difference between revisions of "Part:BBa K3244029"

 
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<partinfo>BBa_K3244029 short</partinfo>
 
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CRISPR HDR for IL-18-producing TRUCK CAR-T Cell  
 
CRISPR HDR for IL-18-producing TRUCK CAR-T Cell  
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[http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have added Part BBa_K3244027 which represents a composite device of 2nd Generation CAR-T, part BBa_K3244028 which represents a composite IL-18 expression construct for TRUCK CAR design and finally BBa_K3244029 which represents  CRISPR Homology Directed Repair template with both L-Arm and R-Arm for IL-18-producing TRUCK CAR-T Cell.
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Results of functional characterization:
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A statistical significance difference was achieved between the % of viability in CAR-T treated BC cells associated Schistosomiasis and unassociated cells (p<0.05). Moreover, between untreated and CAR-t treated BC cells (p<0.01). High reduction of viable BC cells that was treated by CAR-T was demonstrated in Schistosomiasis associated BC compared to unassociated cells, but no statistical significance was achieved (p>0.05).BC cells that was treated with CAR-t cells engineered for ERB2 and Schistosoma egg antigen should a significant apoptosis (54.8%) and 21% dead tumor cells.
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===Usage and Biology===
 
===Usage and Biology===
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For results of functional characterization please refer to our parts Page [https://2019.igem.org/Team:AFCM-Egypt/Parts# EGYPT-AFCM 2019 Parts]
  
 
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Revision as of 22:22, 21 October 2019


CRISPR HDR for IL-18-producing TRUCK CAR-T Cell CRISPR HDR for IL-18-producing TRUCK CAR-T Cell

[http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have added Part BBa_K3244027 which represents a composite device of 2nd Generation CAR-T, part BBa_K3244028 which represents a composite IL-18 expression construct for TRUCK CAR design and finally BBa_K3244029 which represents CRISPR Homology Directed Repair template with both L-Arm and R-Arm for IL-18-producing TRUCK CAR-T Cell. Results of functional characterization: A statistical significance difference was achieved between the % of viability in CAR-T treated BC cells associated Schistosomiasis and unassociated cells (p<0.05). Moreover, between untreated and CAR-t treated BC cells (p<0.01). High reduction of viable BC cells that was treated by CAR-T was demonstrated in Schistosomiasis associated BC compared to unassociated cells, but no statistical significance was achieved (p>0.05).BC cells that was treated with CAR-t cells engineered for ERB2 and Schistosoma egg antigen should a significant apoptosis (54.8%) and 21% dead tumor cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3771
    Illegal XhoI site found at 808
    Illegal XhoI site found at 1630
    Illegal XhoI site found at 2454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3146
    Illegal NgoMIV site found at 6505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6175
    Illegal BsaI.rc site found at 298
    Illegal BsaI.rc site found at 5872
    Illegal SapI site found at 6371