Difference between revisions of "Part:BBa K3317000:Design"

(→‎Source)
(→‎Source)
 
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===Source===
 
===Source===
  
−
From "Sphingobium sp." SYK-6
+
From <i>Sphingobium sp.</i> SYK-6
 
ACCESSION  BAK65949
 
ACCESSION  BAK65949
  
 
===References===
 
===References===
 
Wu, W., Dutta, T., Varman, A. M., Eudes, A., Manalansan, B., Loqué, D., & Singh, S. (2017). Lignin valorization: Two hybrid biochemical routes for the conversion of polymeric lignin into value-added chemicals. Scientific reports, 7(1), 8420.
 
Wu, W., Dutta, T., Varman, A. M., Eudes, A., Manalansan, B., Loqué, D., & Singh, S. (2017). Lignin valorization: Two hybrid biochemical routes for the conversion of polymeric lignin into value-added chemicals. Scientific reports, 7(1), 8420.

Latest revision as of 22:17, 21 October 2019


Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 369
    Illegal NgoMIV site found at 1150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1372


Design Notes

Sequence optimized to e. coli BL21, the ecoRI, XbaI, SpeI and PstI sites were avoided

Source

From Sphingobium sp. SYK-6 ACCESSION BAK65949

References

Wu, W., Dutta, T., Varman, A. M., Eudes, A., Manalansan, B., Loqué, D., & Singh, S. (2017). Lignin valorization: Two hybrid biochemical routes for the conversion of polymeric lignin into value-added chemicals. Scientific reports, 7(1), 8420.