Difference between revisions of "Part:BBa K3192018"

Revision as of 22:17, 21 October 2019

styD

This part contains the gene styD which encodes for the protein phenylacetaldehyde dehydrogenase. This gene was obtained from Pseudomonas putida S12, and combines in conjunction with the overall cluster styABCDE. This is the final step in the degradation of styrene to phenylacetic acid. This catalyzes the oxidation of phenylacetaldehyde to phenylacetic acid.¹

References:

1) Crabo, A. G. et al.Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway. Archives of Biochemistry and Biophysics 616,47–58 (2017).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1380
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 540
Illegal NgoMIV site found at 907
Illegal AgeI site found at 730
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1318

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3192018 short</partinfo>
-This gene encodes for styD, a gene essential for the biochemical pathway degrading styrene into phenyl acetic acid.
+<p>This part contains the gene styD which encodes for the protein phenylacetaldehyde dehydrogenase. This gene was obtained from <i>Pseudomonas putida</i> S12, and combines in conjunction with the overall cluster styABCDE. This is the final step in the degradation of styrene to phenylacetic acid. This catalyzes the oxidation  of phenylacetaldehyde to phenylacetic acid.<sup>1</sup> </p>
+<h3>References: </h3>
+<p> 1) Crabo, A. G. et al.Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway. <i>Archives of Biochemistry and Biophysics</i> 616,47–58 (2017). </p>
 <!-- Add more about the biology of this part here