Difference between revisions of "Part:BBa K3257116"
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This part includes the target gene, which is ChlR_L158X (https://parts.igem.org/Part:BBa_K3257039) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system. | This part includes the target gene, which is ChlR_L158X (https://parts.igem.org/Part:BBa_K3257039) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system. | ||
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| + | [[File:ChlR.jpeg|center|500px|thumb|'''Fig. 1 The verification of well-functional Chl<sup>R</sup> and non-functional Chl<sup>R</sup>_L158X.''' Plasmids containing target gene (Chl<sup>R</sup> and its L158X mutant) and other necessary factors for the reverse transcription and recombination process are transformed into ''E.coli'' BL21(DE3). We can see that cells containing normal Chl<sup>R</sup> are able to grow on a solid plate with chloromphinicol (the right one) while cells containing nonsense mutated Chl<sup>R</sup> cannot grow on a solid plate with chloromphinicol (the left one).]] | ||
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Latest revision as of 21:55, 21 October 2019
T7 Promoter-lox5171-R-PPT-RBS-ChlR_L158X-PBS-U5-R-loxP-T7 Terminator
This part includes the target gene, which is ChlR_L158X (https://parts.igem.org/Part:BBa_K3257039) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1107
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 945
Illegal BsaI.rc site found at 966