Difference between revisions of "Part:BBa K3257111"

Latest revision as of 21:55, 21 October 2019

T7 Promoter-lox5171-R-PPT-RBS-ChlR-PBS-U5-R-loxP-T7 Terminator

This part includes the target gene, which is ChlR (https://parts.igem.org/Part:BBa_K3257034) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination process, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system.

Fig. 1 The verification of well-functional Chl^R and non-functional Chl^R_L158X. Plasmids containing target gene (Chl^R and its L158X mutant) and other necessary factors for the reverse transcription and recombination process are transformed into E.coli BL21(DE3). We can see that cells containing normal Chl^R are able to grow on a solid plate with chloromphinicol (the right one) while cells containing nonsense mutated Chl^R cannot grow on a solid plate with chloromphinicol (the left one).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1107
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 945
Illegal BsaI.rc site found at 966

@@ Line 4: / Line 4: @@
 This part includes the target gene, which is ChlR (https://parts.igem.org/Part:BBa_K3257034) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination process, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system.
-[[File:ChlR.jpeg|center|500px|thumb|'''Fig. 1 The verification of well-functional Chl<sup>R</sup> and non-functional Chl<sup>R</sup>_L158X.''' Plasmids containing target gene (Chl<sup>R</sup> and its L158X mutant) and other necessary factors for the reverse transcription and recombination process are transformed into ''E.coli'' BL21(DE3). We can see that cells containing normal Chl<sup>R</sup> are able to grow on a solid plate with chloromphinicol while cells containing nonsense mutated Chl<sup>R</sup> cannot grow on a solid plate with chloromphinicol.]]
+[[File:ChlR.jpeg|center|500px|thumb|'''Fig. 1 The verification of well-functional Chl<sup>R</sup> and non-functional Chl<sup>R</sup>_L158X.''' Plasmids containing target gene (Chl<sup>R</sup> and its L158X mutant) and other necessary factors for the reverse transcription and recombination process are transformed into ''E.coli'' BL21(DE3). We can see that cells containing normal Chl<sup>R</sup> are able to grow on a solid plate with chloromphinicol (the right one) while cells containing nonsense mutated Chl<sup>R</sup> cannot grow on a solid plate with chloromphinicol (the left one).]]
 <!-- Add more about the biology of this part here