Difference between revisions of "Part:BBa K3304000"
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This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG [[Part:BBa_K3304005]] with initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD [[Part:BBa_K3304006]] and NTB [[Part:BBa_K3304007]] that conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB [[Part:BBa_K3304006]] which constitutes the input for the downstream Join Gate [[Part:BBa_K3304001]]. The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.   
 
This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG [[Part:BBa_K3304005]] with initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD [[Part:BBa_K3304006]] and NTB [[Part:BBa_K3304007]] that conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB [[Part:BBa_K3304006]] which constitutes the input for the downstream Join Gate [[Part:BBa_K3304001]]. The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.   
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Revision as of 21:47, 21 October 2019


Input Gate with NF-κB p65 consensus sequence

This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG Part:BBa_K3304005 with initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD Part:BBa_K3304006 and NTB Part:BBa_K3304007 that conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB Part:BBa_K3304006 which constitutes the input for the downstream Join Gate Part:BBa_K3304001. The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.

T--Thessaloniki--animation-step1.gif T--Thessaloniki--animation-step2.gif T--Thessaloniki--animation-step3.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]