Difference between revisions of "Part:BBa K3244026"
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Gly Ser Linker for TanCAR-T scFvs | Gly Ser Linker for TanCAR-T scFvs | ||
| − | [http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have worked to create an improved Glycine serine linker in part BBa_K1537017 in our new part BBa_K3244026 to adapt for our bispecific extracellular domain which should carry 2 scFv targeting 2 different antigens. This part has been modified through codon optimization -which has represented an issue for our fragment DNA synthesis due to the need for repetition of linker sequence between 4 different chains of 2 scFvs- and amino acid sequence length editing t(GGGGS)5 to act as an inter-chain linker between the 2 scFv for enhanced production and stability of the bispecific scFv. T2A was also added as a basic part in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K3244020# BBa_K3244020]. For results of functional characterization please refer to our parts Page [https://2019.igem.org/Team:AFCM-Egypt/Parts# EGYPT-AFCM 2019 Parts] | + | [http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have worked to create an improved Glycine serine linker in part BBa_K1537017 in our new part BBa_K3244026 to adapt for our bispecific extracellular domain which should carry 2 scFv targeting 2 different antigens. This part has been modified through codon optimization -which has represented an issue for our fragment DNA synthesis due to the need for repetition of linker sequence between 4 different chains of 2 scFvs- and amino acid sequence length editing t(GGGGS)5 to act as an inter-chain linker between the 2 scFv for enhanced production and stability of the bispecific scFv. T2A was also added as a basic part in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K3244020# BBa_K3244020]. For results of functional characterization please refer to our parts Page [https://2019.igem.org/Team:AFCM-Egypt/Parts# EGYPT-AFCM 2019 Parts] Significant difference between GSSS_ BBa_K1537017 engineered and GSSS_BBa_K3244026 T-cells(p<0.05), higher expression levels were observed in GSSS_BBa_K3244026 group. |
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Latest revision as of 21:42, 21 October 2019
Gly Ser Linker for TanCAR-T scFvs
Gly Ser Linker for TanCAR-T scFvs [http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have worked to create an improved Glycine serine linker in part BBa_K1537017 in our new part BBa_K3244026 to adapt for our bispecific extracellular domain which should carry 2 scFv targeting 2 different antigens. This part has been modified through codon optimization -which has represented an issue for our fragment DNA synthesis due to the need for repetition of linker sequence between 4 different chains of 2 scFvs- and amino acid sequence length editing t(GGGGS)5 to act as an inter-chain linker between the 2 scFv for enhanced production and stability of the bispecific scFv. T2A was also added as a basic part in BBa_K3244020. For results of functional characterization please refer to our parts Page EGYPT-AFCM 2019 Parts Significant difference between GSSS_ BBa_K1537017 engineered and GSSS_BBa_K3244026 T-cells(p<0.05), higher expression levels were observed in GSSS_BBa_K3244026 group.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]