Difference between revisions of "Part:BBa K3257075"
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<partinfo>BBa_K3257075 short</partinfo> | <partinfo>BBa_K3257075 short</partinfo> | ||
This part is an orthogonal mutant of loxP and it can only interact with the same lox5171. | This part is an orthogonal mutant of loxP and it can only interact with the same lox5171. | ||
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| + | [[File:Cre+lox Sites.png|center|500px|thumb|'''Fig. 1 The verification of incompatibility between LoxP and different Lox sites.''' Plasmids containing the Cre gene were co-transformed respectively with plasmids containing mCherry flanked by a LoxP site and either LoxP, Lox2272 or Lox5171 site. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only. ]] | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K3257075 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3257075 SequenceAndFeatures</partinfo> | ||
Latest revision as of 21:41, 21 October 2019
lox5171
This part is an orthogonal mutant of loxP and it can only interact with the same lox5171.
Fig. 1 The verification of incompatibility between LoxP and different Lox sites. Plasmids containing the Cre gene were co-transformed respectively with plasmids containing mCherry flanked by a LoxP site and either LoxP, Lox2272 or Lox5171 site. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]