Difference between revisions of "Part:BBa K3257074"

Revision as of 21:40, 21 October 2019

lox2272

This part is a binding site for Cre recombinase and it is orthogonal to the native loxP site which means that it can only interact with the same lox2272 site rather than loxP.

Fig. 1 The verification of incompatibility between LoxP and different Lox sites. Plasmids containing the Cre-SRC gene were co-transformed respectively with plasmids containing mCherry flanked by a LoxP site and either LoxP, Lox2272 or Lox5171 site. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre-SRC gene only.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part is a binding site for Cre recombinase and it is orthogonal to the native loxP site which means that it can only interact with the same lox2272 site rather than loxP.
-[[File:Cre+lox Sites.png|center|500px|thumb|'''Figure 2. The verification of DNA cleavage between LoxP sites.''' Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only. ]]
+[[File:Cre+lox Sites.png|center|500px|thumb|'''Fig. 1 The verification of incompatibility between LoxP and different Lox sites.''' Plasmids containing the Cre-SRC gene were co-transformed respectively with plasmids containing mCherry flanked by a LoxP site and either LoxP, Lox2272 or Lox5171 site. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre-SRC gene only. ]]
 <!-- Add more about the biology of this part here