Difference between revisions of "Part:BBa K2984012"
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==References==
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Berthold, P., Schmitt, R., & Mages, W. (2002). An engineered Streptomyces hygroscopicus aph 7" gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist, 153(4), 401-412.
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Revision as of 21:38, 21 October 2019


Hygromycin B CDS; Selection for Transformation - Antibiotic resistance aphVII (hyg) B3-B4

This part is a part of the Chlamy-HUB-Collection. Berthold et al. (2002)[1] found that the aminoglycoside phosphotransferase gene (aphVII) from Streptomyces hygroscopicus works as a selectable marker for C. reinhardtii establishing a hygromycin resistance.

This part was designed to be used with the MoClo standard and has B3-B4 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 797
    Illegal NgoMIV site found at 905
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Plate_L0-Hyg_B3-B4_E.coli
Fig.1 - Successful transformation of L0-Hyg_B3-B4 into E. coli.


References

  1. Berthold, P., Schmitt, R., & Mages, W. (2002). An engineered Streptomyces hygroscopicus aph 7" gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist, 153(4), 401-412.