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===Usage and Biology===
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===Characterization===
  
 
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Revision as of 21:37, 21 October 2019


Quorum-Sensing Receiver Construct

Transformed cells with this construct respond to AHL-dependent quorum sensing and GFP would be expressed by the activated transcription factor protein LuxR which binds to the bidirectional promoter region (pLuxR/pLuxI).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1661