Difference between revisions of "Part:BBa K3257065"
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<partinfo>BBa_K3257065 short</partinfo> | <partinfo>BBa_K3257065 short</partinfo> | ||
| − | This part is an orthogonal mutant of loxP and it can only interact with the same lox511. | + | This part is an orthogonal mutant of loxP and it is expected that it can only interact with the same lox511 while our results show that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency. |
[[File:Cre+lox511.png|center|500px|thumb|'''Figure 1. lox511 remains compatible with wildtype loxP, though at a lower excision rate.''' Wildtype loxP and lox511-mCherry-loxP are analyzed on two different gels, their marker bands are indicated. Wildtype loxP only has an excision band. lox511 has a slight full-length mCherry band slightly longer than 1000 bp, but excision band is still visible and brighter than that of full-length mCherry. This result suggests that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency.]] | [[File:Cre+lox511.png|center|500px|thumb|'''Figure 1. lox511 remains compatible with wildtype loxP, though at a lower excision rate.''' Wildtype loxP and lox511-mCherry-loxP are analyzed on two different gels, their marker bands are indicated. Wildtype loxP only has an excision band. lox511 has a slight full-length mCherry band slightly longer than 1000 bp, but excision band is still visible and brighter than that of full-length mCherry. This result suggests that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency.]] | ||
Latest revision as of 21:35, 21 October 2019
lox511
This part is an orthogonal mutant of loxP and it is expected that it can only interact with the same lox511 while our results show that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency.
Figure 1. lox511 remains compatible with wildtype loxP, though at a lower excision rate. Wildtype loxP and lox511-mCherry-loxP are analyzed on two different gels, their marker bands are indicated. Wildtype loxP only has an excision band. lox511 has a slight full-length mCherry band slightly longer than 1000 bp, but excision band is still visible and brighter than that of full-length mCherry. This result suggests that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]