Difference between revisions of "Part:BBa K3257065"
| Line 4: | Line 4: | ||
This part is an orthogonal mutant of loxP and it can only interact with the same lox511. | This part is an orthogonal mutant of loxP and it can only interact with the same lox511. | ||
| + | [[File:Cre+lox511.png|center|500px|thumb|'''Figure 1. lox511 remains compatible with wildtype loxP, though at a lower excision rate.''' Wildtype loxP and lox511-mCherry-loxP are analyzed on two different gels, their marker bands are indicated. Wildtype loxP only has an excision band. lox511 has a slight full-length mCherry band slightly longer than 1000 bp, but excision band is still visible and brighter than that of full-length mCherry. This result suggests that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency.]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 21:34, 21 October 2019
lox511
This part is an orthogonal mutant of loxP and it can only interact with the same lox511.
Figure 1. lox511 remains compatible with wildtype loxP, though at a lower excision rate. Wildtype loxP and lox511-mCherry-loxP are analyzed on two different gels, their marker bands are indicated. Wildtype loxP only has an excision band. lox511 has a slight full-length mCherry band slightly longer than 1000 bp, but excision band is still visible and brighter than that of full-length mCherry. This result suggests that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]