Difference between revisions of "Part:BBa K3244026"

Revision as of 21:29, 21 October 2019

Gly Ser Linker for TanCAR-T scFvs

Gly Ser Linker for TanCAR-T scFvs [http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have worked to create an improved Glycine serine linker in part BBa_K1537017 in our new part BBa_K3244026 to adapt for our bispecific extracellular domain which should carry 2 scFv targeting 2 different antigens. This part has been modified through codon optimization -which has represented an issue for our fragment DNA synthesis due to the need for repetition of linker sequence between 4 different chains of 2 scFvs- and amino acid sequence length editing t(GGGGS)5 to act as an inter-chain linker between the 2 scFv for enhanced production and stability of the bispecific scFv. T2A was also added as a basic part in BBa_K3244020. For reulsults of functional characterization please refer to our parts Page EGYPT-AFCM 2019 Parts

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Gly Ser Linker for TanCAR-T scFvs
+[http://2019.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2019] have worked to create an improved Glycine serine linker in part BBa_K1537017 in our new part BBa_K3244026 to adapt for our bispecific extracellular domain which should carry 2 scFv targeting 2 different antigens. This part has been modified through codon optimization -which has represented an issue for our fragment DNA synthesis due to the need for repetition of linker sequence between 4 different chains of 2 scFvs- and amino acid sequence length editing t(GGGGS)5 to act as an inter-chain linker between the 2 scFv for enhanced production and stability of the bispecific scFv. T2A was also added as a basic part in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K3244020# BBa_K3244020]. For reulsults of functional characterization please refer to our parts Page [https://2019.igem.org/Team:AFCM-Egypt/Parts# EGYPT-AFCM 2019 Parts]
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