Difference between revisions of "Part:BBa K3257064"
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'''Prove of well-functional LoxP sites''' | '''Prove of well-functional LoxP sites''' | ||
| − | [[File:Cre+loxP.png|center|500px|thumb|'''Figure | + | [[File:Cre+loxP.png|center|500px|thumb|'''Figure 1. The verification of DNA cleavage between LoxP sites.''' Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only. ]] |
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Latest revision as of 21:28, 21 October 2019
loxP
The loxP sites are recognized by Cre recombinase to initiate the recombination process.
Prove of well-functional LoxP sites
Figure 1. The verification of DNA cleavage between LoxP sites. Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]