Difference between revisions of "Part:BBa K3304003"

Revision as of 21:27, 21 October 2019

Reporter complex for output - TCC

This is the reporter complex that generates the fluorescent output to be measured as a quantitative readout of the formal CRN's function. The top strand is labeled with a fluorophore molecule at its 3' end while it's complementary strand is labeled with a quencher at it's 5' end. The quencher is able to absorb the fluorescence emitted by the fluorophore for as long as they're paired. The strand displacement reaction induces the separation of the quencher from the fluorophore producing measurable output.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3304003 short</partinfo>
-This is the reporter complex that generates the fluorescent output to be measured as a quantitative readout of the formal CRN's function. The top strand is labeled with a quencher molecule at its 3' end while it's complementary strand is labeled with a fluorescent molecule at it's 5' end. The strand displacement reaction separates the quencher from the fluorophore molecule producing measurable output.
+This is the reporter complex that generates the fluorescent output to be measured as a quantitative readout of the formal CRN's function. The top strand is labeled with a fluorophore molecule at its 3' end while it's complementary strand is labeled with a quencher at it's 5' end. The quencher is able to absorb the fluorescence emitted by the fluorophore for as long as they're paired. The strand displacement reaction induces the separation of the quencher from the fluorophore producing measurable output.
 https://2019.igem.org/wiki/images/d/d3/T--Thessaloniki--animation-step11.gif