Difference between revisions of "Part:BBa K3257122"

Latest revision as of 21:26, 21 October 2019

mCherry with J23001 Promoter

This part is an expression system for the red fluorescence protein, mCherry.

Prove of well-functional mCherry

Figure 1. The relative fluorescence intensity of mCherry and its mutants mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside E.coli cells.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K3257122 short</partinfo>
 This part is an expression system for the red fluorescence protein, mCherry.
+'''Prove of well-functional mCherry'''
+[[File:MCherry and its mutants.png|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of mCherry and its mutants''' mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X  represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside ''E.coli'' cells.]]
 <!-- Add more about the biology of this part here
@@ Line 9: / Line 12: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'''Sequence and Features'''</span>
 <partinfo>BBa_K3257122 SequenceAndFeatures</partinfo>