Difference between revisions of "Part:BBa K1692032"
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This plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002. For more information about this gene, please refer to the <partinfo>BBa_K592009</partinfo> part page. | This plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002. For more information about this gene, please refer to the <partinfo>BBa_K592009</partinfo> part page. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p>The chromoprotein amilCP is part of a family of GFP-like fluorescent proteins derived from reef-building corals of the class Anthozoa (Alieva et al., 2008). Similar to the GFP family of proteins, the β-barrel of amilCP encloses a chromophore (Tafoya-Ramírez et al., 2018). Along with other coral chromoproteins, amilCP is non-fluorescent and forms a tetramer, resulting in a fairly stable protein structure (Alieva et al., 2008; Tafoya-Ramírez et al., 2018). These GFP-like fluorescent proteins give corals their vivid and varied colors (Alieva et al., 2008). </p> | <p>The chromoprotein amilCP is part of a family of GFP-like fluorescent proteins derived from reef-building corals of the class Anthozoa (Alieva et al., 2008). Similar to the GFP family of proteins, the β-barrel of amilCP encloses a chromophore (Tafoya-Ramírez et al., 2018). Along with other coral chromoproteins, amilCP is non-fluorescent and forms a tetramer, resulting in a fairly stable protein structure (Alieva et al., 2008; Tafoya-Ramírez et al., 2018). These GFP-like fluorescent proteins give corals their vivid and varied colors (Alieva et al., 2008). </p> | ||
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<p>Tafoya-Ramírez, M. D., Padilla-Vaca, F., Ramírez-Saldaña, A. P., Mora-Garduño, J. D., Rangel-Serrano, Á., Vargas-Maya, N. I., … Franco, B. (2018). Replacing Standard Reporters from Molecular Cloning Plasmids with Chromoproteins for Positive Clone Selection. Molecules (Basel, Switzerland), 23(6). https://doi.org/10.3390/molecules23061328 </p> | <p>Tafoya-Ramírez, M. D., Padilla-Vaca, F., Ramírez-Saldaña, A. P., Mora-Garduño, J. D., Rangel-Serrano, Á., Vargas-Maya, N. I., … Franco, B. (2018). Replacing Standard Reporters from Molecular Cloning Plasmids with Chromoproteins for Positive Clone Selection. Molecules (Basel, Switzerland), 23(6). https://doi.org/10.3390/molecules23061328 </p> | ||
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Revision as of 21:26, 21 October 2019
amilCP blue chromoprotein with RBS and promoter
This plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002. For more information about this gene, please refer to the BBa_K592009 part page.
Usage and Biology
The chromoprotein amilCP is part of a family of GFP-like fluorescent proteins derived from reef-building corals of the class Anthozoa (Alieva et al., 2008). Similar to the GFP family of proteins, the β-barrel of amilCP encloses a chromophore (Tafoya-Ramírez et al., 2018). Along with other coral chromoproteins, amilCP is non-fluorescent and forms a tetramer, resulting in a fairly stable protein structure (Alieva et al., 2008; Tafoya-Ramírez et al., 2018). These GFP-like fluorescent proteins give corals their vivid and varied colors (Alieva et al., 2008).
Isolated from the species Acropora millepora, amilCP was first described in 2008, and is characterized by a strong color due to its very high molar extinction coefficient of 87 600 (Alieva et al., 2008). Its maximum excitation wavelength is 588nm (Alieva et al., 2008). Interestingly, amilCP’s max absorption is shifted into the red spectrum by ~10nm (592nm), hence appearing more blue than purple to the naked eye (Alieva et al., 2008). Such blue color is due to 2 mutations resulting in amino acid substitutions (Alieva et al., 2008).
References
Alieva, N. O., Konzen, K. A., Field, S. F., Meleshkevitch, E. A., Hunt, M. E., Beltran-Ramirez, V., … Matz, M. V. (2008). Diversity and evolution of coral fluorescent proteins. PLoS ONE, 3(7). https://doi.org/10.1371/journal.pone.0002680
Tafoya-Ramírez, M. D., Padilla-Vaca, F., Ramírez-Saldaña, A. P., Mora-Garduño, J. D., Rangel-Serrano, Á., Vargas-Maya, N. I., … Franco, B. (2018). Replacing Standard Reporters from Molecular Cloning Plasmids with Chromoproteins for Positive Clone Selection. Molecules (Basel, Switzerland), 23(6). https://doi.org/10.3390/molecules23061328
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]