Difference between revisions of "Part:BBa K3257047"

This part is a truncated mCherry and it only contains amino acid residues from the1^st to the 158^th of wild-type mCherry so that it cannot give out red light when stimulated. We use this part to prove the ability of Cre's homologous recombination. When there is a Cre recombinase present, a wild-type mCherry will be replaced by this truncated mCherry and red fluorescence disappears.

This part is a truncated mCherry and it only contains amino acid residues from the1^st to the 158^th of wild-type mCherry so that it cannot give out red light when stimulated. We use this part to prove the ability of Cre's homologous recombination. When there is a Cre recombinase present, a wild-type mCherry will be replaced by this truncated mCherry and red fluorescence disappears.

[[File:MCherry and its mutants.png|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of mCherry and its mutants''' mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X  represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside ''E.coli'' cells.]]

[[File:MCherry and its mutants.png|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of mCherry and its mutants''' mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X  represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside ''E.coli'' cells.]]