Difference between revisions of "Part:BBa K3183001:Design"
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<partinfo>BBa_K3183001 short</partinfo> | <partinfo>BBa_K3183001 short</partinfo> | ||
| − | The part was identified in the article by Lizier et al; the gene was codon optimised for <i>L. reuteri</i> and synthesized by IDT. The part was leter assembled by Gibson Assembly into BBa_K3183102, which was finally assembled into the pTRKH3 vector BBa_K3183050, again by Gibson Assembly | + | The part was identified in the article by Lizier et al; the gene was codon optimised for <i>L. reuteri</i> and synthesized by IDT. The part was leter assembled by Gibson Assembly into <partinfo>BBa_K3183102</partinfo>, which was finally assembled into the pTRKH3 vector (<partinfo>BBa_K3183050</partinfo>), again by Gibson Assembly |
<partinfo>BBa_K3183001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3183001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 21:23, 21 October 2019
Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.
The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into BBa_K3183102, which was finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23, RF25, and RF1000 standards
Source
It is derived from the L. acidophilus surface layer protein promoter.
References
Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.