Difference between revisions of "Part:BBa K3257033"
| Line 4: | Line 4: | ||
This part is a red fluorescence protein mCherry. We added loxP sites at both ends of it to test if Cre recombinase worked properly. | This part is a red fluorescence protein mCherry. We added loxP sites at both ends of it to test if Cre recombinase worked properly. | ||
| − | [[File:MCherry and its mutants.png|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of mCherry and its mutants''' mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry.]] | + | [[File:MCherry and its mutants.png|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of mCherry and its mutants''' mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside ''E.coli'' cells.]] |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 21:21, 21 October 2019
mCherry
This part is a red fluorescence protein mCherry. We added loxP sites at both ends of it to test if Cre recombinase worked properly.
Figure 1. The relative fluorescence intensity of mCherry and its mutants mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside E.coli cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]