Difference between revisions of "Part:BBa K3046014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | This part is created on the basis of the upstream region of the sonB ORF (Systematic ORF name: An11g02200) of many <i>aspergillus</i> genomes.It has been created using the proHMMoter software, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model | |
===References=== | ===References=== | ||
| + | [1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019. | ||
Latest revision as of 21:13, 21 October 2019
PLEAPunk_4
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 42
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 402
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
Source
This part is created on the basis of the upstream region of the sonB ORF (Systematic ORF name: An11g02200) of many aspergillus genomes.It has been created using the proHMMoter software, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
References
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.