Difference between revisions of "Part:BBa K3122000"
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<h2> Characterization </h2> | <h2> Characterization </h2> | ||
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[[File:https://2019.igem.org/wiki/images/d/d3/T--MADRID_UCM--wp-content~uploads~2019~10~nada_2.png|900px|thumb|none|alt=Virus assay petri dish.|Figure 1. ]] | [[File:https://2019.igem.org/wiki/images/d/d3/T--MADRID_UCM--wp-content~uploads~2019~10~nada_2.png|900px|thumb|none|alt=Virus assay petri dish.|Figure 1. ]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2656000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2656000 SequenceAndFeatures</partinfo> | ||
Revision as of 21:12, 21 October 2019
No part name specified with partinfo tag. E. coli LamB is one of the best characterized and most flexible heterologous peptide carriers. This is due to its extracelular domains, consisting mainly of long loops. One of these loops can tolerate insertions of rather long sequences, between amino acid residues 153 and 154 (Sousa, 1996).
Our group have adapted this functional display system to type IIS enzymes grammar, and called it AEGIS's lamB display system for Gram-negative bacteria. This part was designed to use the outer membrane protein lamB of E. coli K12 strain to express any tag or short sequence to the extracellular medium on its specific permissive loop. The advantages of this approach are notable: not only can it be used for bacteria with cell wall ensuring its right functioning, but also it is really simple and easy to use following MoClo standard.
To see the design of this part go to the Design tab.
Biology
LamB is maltoporin precursor of Gram-negative bacteria. This outer membrane proteins family is involved in transport of maltodextrins across the outer membrane. Besides, LamB is the receptor of several different bacteriophagues as lambda fague. Gene sequence of LamB [Escherichia coli str. K-12 substr. MG1655] was obtained from EcoGene:EG10528.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]