Difference between revisions of "Part:BBa K3257032"

Latest revision as of 21:10, 21 October 2019

EGFP_K159X

This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 159th amino acid residue. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing.

Figure 1. The relative fluorescence intensity of EGFP and its nonsense mutants EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. EGFP_Q158X and EGFP_K159X represents the nonsense mutants of wild-type EGFP. According to the relative fluorescence intensity, we can see that EGFP nonsense mutants lose the ability of fluorescing.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3257032 short</partinfo>
-This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 159th amino acid residual. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing.
+This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 159th amino acid residue. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing.
+[[File:EGFP Mutants.png|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of EGFP and its nonsense mutants''' EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. EGFP_Q158X and EGFP_K159X represents the nonsense mutants of wild-type EGFP. According to the relative fluorescence intensity, we can see that EGFP nonsense mutants lose the ability of fluorescing.]]
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